Self-incompatibility characterization in segregating populations of apple trees with DNA markers for S-alleles

Abstract The objective of this study was to characterize the parents and respective populations apple trees regarding S-alleles confirm their genealogy evaluate efficiency molecular markers used. Sixteen specific sets primers were used for identification by PCR. Two segregating Epagri Apple Breeding Program resulting from crosses between ‘Fred Hough’ × ‘Monalisa’ ‘M-11/00’ ‘M-13/91’ evaluated. expected segregations are 1:1:1:1 full compatibility 1:1 semi-compatibility, which can be confirmed X2 test. (S5S19) (S2S10) cross proved fully compatible; two triploids identified among hybrids as well. (S3S19) (S3S5) characterized semi-compatible based on DNA markers, segregation in 1:1, expected. occurred together with S-alleles: S2, S3, S5, S10, S19. Thus, characterization not only allowed but also contaminations populations.